Picking a CryoEM Provider: Your Checklist for 2025

Selecting the right CryoEM Services provider is a critical decision for researchers aiming to visualize biomolecules at high resolution. As we approach 2025, the landscape of structural biology is rapidly evolving, with advancements in technology and increasing demand for detailed structural insights. This checklist will guide you through the key considerations when choosing a CryoEM Services partner, ensuring your project achieves its goals efficiently and effectively. For more comprehensive information on our CryoEM Services, please visit https://shuimubio.com/ and contact our marketing team for further inquiries.

1. Comprehensive Service Portfolio:

• Does the provider offer a one-stop SPA (Single Particle Analysis) solution? Look for a provider that can handle a diverse range of samples, including antigen-antibody complexes, small molecules & targets, PROTACs, membrane proteins (GPCRs, ion channels, transporters), VLPs, peptides, etc.. Shuimu BioSciences offers such comprehensive one-stop SPA solutions.
• Are various grid types available to address challenging samples? Consider providers offering advanced support grids like GraFuture™, GO (Graphene Oxide) and RGO (Reduced Graphene Oxide), which can help overcome issues such as small protein molecular weight, low concentration, high background noise, air-water interface disruption, and preferential orientation. Shuimu BioSciences has developed these GraFuture™ series of graphene support grids.
• Do they provide Cryo Characterization services for samples like AAV, liposomes, LNPs, and VLPs? This is crucial for understanding the quality and homogeneity of your samples before high-resolution data collection. Shuimu BioSciences offers efficient and precise cryo-characterization services. Our self-developed AI system, NanoSMART, can automatically identify nanoparticle features and provide detailed reports.
• Is MicroED (Micro-electron Diffraction) available for structure determination of small molecule drugs and protein crystals? This cutting-edge technology is essential for samples that are challenging for traditional cryo-EM SPA. Shuimu BioSciences excels in providing accurate structural insights using MicroED technology. Our proprietary eTasED software integrates MicroED into conventional cryo-EM systems.
• Are Negative Staining and 2D Negative Staining services offered for initial assessment of sample homogeneity and observation of tissue sections? This lower-resolution technique can provide valuable preliminary data and insights. Shuimu BioSciences provides leading negative staining technology for quick initial assessments.

For a complete overview of our CryoEM Services, please visit https://shuimubio.com/ and contact our marketing team for further inquiries.

2. Cutting-Edge Instrumentation and Technology:

• Does the provider have state-of-the-art cryo-electron microscopes? Look for 300 kV data acquisition capabilities. Shuimu BioSciences operates a large commercial cryo-EM platform with 300 kV instruments in Beijing and Hangzhou.
• Is 24-hour instrument access available? This can significantly accelerate your data collection timeline. Shuimu BioSciences provides 24-hour, round-the-clock instrument time.
• Are advanced hardware configurations in place, including high-performance detectors, energy filters, spherical aberration, and phase plates? These features are crucial for maximizing imaging quality. Shuimu BioSciences’s Electron Microscopy Center is equipped with the world’s most advanced biomolecular imaging equipment.
• Is an AI-driven platform used to streamline data analysis? AI can enhance efficiency and accuracy in structure determination. Shuimu BioSciences has independently developed the SMART software suite utilizing AI to streamline cryo-EM data analysis.

To learn more about our advanced instrumentation for CryoEM Services, please visit https://shuimubio.com/ and contact our marketing team for further inquiries.

3. Experienced Scientific Team:

• Is the team composed of PhD-level experts in structural biology, protein science, and computational biology? Experienced scientists are essential for project consultation, strategy definition, and data interpretation. Shuimu BioSciences’s core team comprises world-class experts and our scientist team includes PhD-level experts from preeminent institutions.
• What is their track record in resolving high-resolution structures? Inquire about the number of completed projects and the best resolutions achieved. Shuimu BioSciences has completed over 400 cryo-EM projects, with more than 150 structures resolved, achieving a best resolution of 1.8 Å. We have successfully elucidated protein structures as small as 51kDa.
• Do they offer expert support throughout the entire workflow, from sample preparation to data delivery? A dedicated team ensures optimal performance and addresses any unexpected situations. Shuimu BioSciences has established efficient, advanced workflows and a dedicated team of EM engineers oversees daily operations.

Meet our elite scientist team by visiting https://shuimubio.com/ and contact our marketing team for further inquiries.

4. Comprehensive Experimental Workflow and Support:

• Do they offer support for protein preparation and analysis, including protein expression systems, purification processes, and quality control? Integrating upstream capabilities minimizes variability and addresses challenges with difficult-to-express proteins. Shuimu BioSciences has built a complete protein expression and purification platform.
• Is project feasibility assessment offered? This complimentary service can help you understand the potential and risks of your project. Shuimu BioSciences offers a free complimentary project feasibility assessment.
• Are there regular meetings to track progress? Clear communication is crucial for a successful collaboration. Shuimu BioSciences ensures regular meetings to track progress.
• What are the sample submission requirements? Ensure your samples meet the necessary quality and quantity criteria. Shuimu BioSciences provides detailed sample submission requirements for various services.
• What is the typical data delivery timeline? Efficient data processing and delivery are essential for timely research outcomes.

Review our detailed experimental workflow at https://shuimubio.com/ and contact our marketing team for further inquiries regarding project timelines.

5. Proven Track Record and Applications:

• Has the provider contributed to significant research outcomes published in top international journals? This demonstrates their expertise and the quality of their services. Numerous research results supported by Shuimu BioSciences’s platform have been published in top international journals, covering ion channels, GPCRs, antigen-antibody complexes, and more.
• Do they have experience with a wide range of application scenarios, including analyzing biomacromolecules, vaccine development, and antibody drug discovery? Understanding their application expertise ensures they can cater to your specific research needs. Shuimu BioSciences has extensive experience in analyzing various biomacromolecules, supporting vaccine R&D and quality control, and contributing to antibody drug development.

Explore our successful case sharing examples at https://shuimubio.com/ and contact our marketing team for more information on relevant applications.

By carefully considering these factors and using this checklist, you can confidently select the CryoEM Services provider that best aligns with your research objectives for 2025. Remember to visit https://shuimubio.com/ for a comprehensive understanding of Shuimu BioSciences’s offerings and contact our marketing team for any inquiries.